Auxin Inducible Degron System
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Auxin inducible degron system. To apply AID an auxin-inducible destabilizing domain or degron is fused to. 5 The auxin-inducible degron AID technology which utilizes plant auxin signaling 6 components to control protein degradation in non-plant species is a widely used 7 small-molecular-controlled degradation method in yeasts and animals. The auxin-inducible degron AID technology which utilizes plant auxin signaling components to control protein degradation in non-plant species is a widely used small-molecular-controlled degradation method in yeasts and animals.
In human cells expressing an E3 ligase component OsTIR1 it is possible to degrade a degron-fused protein with a half-life of 1545 min in the presence of the phytohormone auxin. Known degrons include short amino acid sequences structural motifs and exposed amino acids often Lysine or Arginine located anywhere in the proteinIn fact some proteins can even contain multiple degrons. Fusion of inducible degradation signals so-called degrons to cellular proteins is an elegant method of controlling protein levels in vivo.
This method can conditionally induce the degradation of any protein by the proteasome simply by the addition of the plant hormone auxin. We pioneered the development of the auxin-inducible degron AID technology by transplanting a plant-specific degradation pathway to non-plant cells. Principles of the auxin-inducible degron system.
This system allowed us to induce rapid depletion Of a protein Of interest within 30 min in the presence Of auxin and the reaction was reversible and tunable. Auxin-inducible degron AID technology allows rapid targeted protein depletion with the small-molecule auxin 2. The auxin-inducible degron AID system enables rapid depletion of target proteins within the cell by applying the natural auxin IAA.
This approach is particularly valuable to study the function of essential proteins. Auxin inducible degron AID system is used for the targeted protein degradation and can be applied to the mammalian cells Nishimura et al 2009. The auxin-inducible degron AID system has emerged as a powerful tool to conditionally deplete proteins in a range of organisms and cell types.
The auxin-inducible degron AID is a useful technique to rapidly deplete proteins of interest in nonplant eukaryotes. However the currently available AID systems still have room for further optimization. An expanded tool kit for the auxin-inducible degron system in budding yeast.
The auxin-inducible degron AID is a powerful tool that is used for depletion of proteins to study their function in vivo. A degron is a portion of a protein that is important in regulation of protein degradation rates.
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The auxin-inducible degron AID is a powerful tool that is used for depletion of proteins to study their function in vivo.
This approach is particularly valuable to study the function of essential proteins. Here we establish an improved AID system using an engineered orthogonal auxin-TIR1 pair which exhibits over 1000 times stronger binding. To apply AID an auxin-inducible destabilizing domain or degron is fused to. The auxin-inducible degron AID technology which utilizes plant auxin signaling components to control protein degradation in non-plant species is a widely used small-molecular-controlled degradation method in yeasts and animals. The auxin-inducible degron AID system was developed as a tool to achieve rapid and inducible protein degradation in nonplant systems. This system worked in budding yeast as well as in cell lines derived from chicken mouse hamster monkey and human. Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUXIAA family of transcription repressors by a specific form of the SCF E3 ubiquitin ligase. This method can conditionally induce the degradation of any protein by the proteasome simply by the addition of the plant hormone auxin. However the applicability of the AID in vertebrate cells has been limited due to cytotoxicity caused by high auxin concentrations.
Auxin-inducible degron AID technology allows rapid targeted protein depletion with the small-molecule auxin 2. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin-inducible degron AID system in Saccharomyces cerevisiae. The auxin-inducible degron AID is a powerful tool that is used for depletion of proteins to study their function in vivo. An expanded tool kit for the auxin-inducible degron system in budding yeast. However this system generally requires high dose of auxin to achieve effective depletion in vertebrate cells. This method can conditionally induce the degradation of any protein by the proteasome simply by the addition of the plant hormone auxin. Principles of the auxin-inducible degron system.
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